Evaluation of A Reverse Transcriptase (Rt) Loop Mediated Isothermal Amplification Assay for Detection of Hepatitis C Genotypes 1-4 Viruses Under Limited Logistical Conditions
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چکیده
منابع مشابه
Evaluation of A Reverse Transcriptase (Rt) Loop Mediated Isothermal Amplification Assay For Detection Hepatitis C Genotypes 1-4 Viruses Under Limited Logistical Conditions
Hepatitis C Virus remains a major public health concern in many parts of the world due to its long sero-conversion period presenting it as a high risk in transfusion related transmission. The transfusion related transmission still remains a concern in many developing countries and resource related settings [1] which are highly dependent on serological tests for diagnosis and routine screening o...
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Bovine viral diarrhea virus (BVDV) is a pathogen that infects cattle, and is globally important. It causes substantial financial losses to the livestock industry. In the current study, a one-step reverse transcriptase-loop-mediated isothermal amplification (RT-LAMP) assay was set up for rapid and efficient detection of BVDV. For this purpose, four primers were designed to recognize six distinct...
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The one-step single-tube betaine-free reverse transcription (RT)-loop-mediated isothermal amplification assay was developed for rapid diagnosis of hepatitis E virus. This assay amplified the target gene in less than 45 min (even as short as 20 min) under isothermal conditions at 63 degrees C, and the sensitivity of this assay was 100-fold greater than that of RT-PCR. This assay demonstrated a d...
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ژورنال
عنوان ژورنال: Journal of Human Virology & Retrovirology
سال: 2017
ISSN: 2373-6453
DOI: 10.15406/jhvrv.2017.05.00145